The ROTI^®Mark WESTERN PLUS protein ladder covers a wide range of molecular weights from 15 to 200 kDa. It consists of 10 IgG binding proteins with two in their mass enhanced reference bands (30 kDa and 80 kDa). For the most common type of primary antibodies are IgG type antibodies the ROTI^®Mark WESTERN PLUS ladder can be used in almost any situation of Western Blot. Appropriate detection methods are fluorescence, chemoluminescence, colour or other systems.

For controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot the ladder additionally contains four prestained proteins: 10 kDa (blue), 25 kDa (green), 45 kDa (blue) und 70 kDa (red).

Fig.: Marker after detection on X-ray film (left), after Western blot on PVDF membrane (right)