TREX1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91372-50, BS91372-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NSU2(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IF, IHC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: TREX1 polyclonal antibody detects endogenous levels of TREX1 protein.

Bioworld Molecular Weight: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TREX1 on different cell lysates using anti-TREX1 at 1/500 dilution.
Positive control:
Line 1: A431
Line 2: SK-Br-3, ICC staining TREX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: DNase III, TREX1, 3' 5' exonuclease TREX1, 3' repair exonuclease 1, AGS1, AGS5, CRV, Deoxyribonuclease III, dnaQ/mutD (E. coli) like, DKFZp434J0310, DNase III, DRN3, HERNS, Three prime repair exonuclease 1, TREX1,

Immunogen: Recombinant protein within human TREX1 aa 100-300.

Conjugate: Unconjugated

Modification: Unmodified