CA II Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9113-50, MB9113-100

Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P00918 Human;P00920 Mouse;P27139 Rat

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: CA II monoclonal antibody detects endogenous levels of CA II protein.

Bioworld Molecular Weight: 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Carbonic anhydrase 2 on different cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: HL-60 cell lysate, ICC staining Carbonic anhydrase 2 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carbonic anhydrase 2 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Alternative Name: CAC, CA-II, CA2, can, CAN_ECOLI, Carbonate dehydratase 2, Carbonic anhydrase 2 antibody

Immunogen: Recombinant protein within Human Carbonic anhydrase 2 aa 50-220.

Conjugate: Unconjugated

Modification: Unmodified