PBR Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9815M-50, BS9815M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P30536

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF, IHC

All Applications: WB: 1:1000-1:5000
IHC/IF: 1:50-1:200

Background: Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR.

Purification and Purity: Affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of PBR and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using PBR Rabbit mAb at 1:1000 dilution., mmunohistochemistry of paraffin-embedded human colon using PBR Rabbit mAb at dilution of 1:100 (40x lens)., Immunofluorescence analysis of NIH-3T3 cells using PBR Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using PBR Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Alternative Name: Translocator protein; Mitochondrial benzodiazepine receptor; PKBS; Peripheral-type benzodiazepine receptor; TSPO; BZRP; MBR

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified