Galectin 3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90556-50, BS90556-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17931(Human) P08699(Rat)

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, ICC/IF FC

All Applications: WB:1:1,000-1:5,000
FC:1:50-1:100
ICC:1:50-1:200

Background: Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) inter- actions and play a role in tumor progression, pre-mRNA splicing and apop-tosis. The galectin-3 protein, also known as Mac-2, hMac-2, GALBP, CBP35 or LGALS3, contains a single carbohydrate binding domain, which binds galactose-containing glycoconjugates. Galectin-3 is expressed in colonic and intestinal epithelium, inflammatory macrophages, papillary and follicular carcinomas, neoplastic astrocytes and some B and T lymphocytes. Upregulated expression of galectin-3 is involved in cancer progression and metastasis. Galectin-3 mediates the endocytosis of β1 Integrins in a lactose-dependent manner and is associated with thyroid malignancy and Crohn's disease. It may also be used as a marker for diagnosing cases involving Hurthle cell adenomas and carcinomas.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Galectin 3 polyclonal antibody detects endogenous levels of Galectin 3 protein.

Bioworld Molecular Weight: 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Galectin 3 on different lysates using anti-Galectin 3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: THP-1, ICC staining Galectin 3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Gal-3, CBP 35, GALBP, LGALS3, 35 kDa lectin, Carbohydrate binding protein 35, Carbohydrate-binding protein 35, CBP 35, CBP35, Gal-3, GAL3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, Galectin 3 internal gene, included, Galectin-3, Galectin3, GALIG, GBP, IgE binding protein, IgE-binding protein, L 31, L 34, L-31, L-34 galactoside-binding lectin, L31, Laminin-binding protein, Lectin L-29, Lectin, galactose binding, soluble 3, LEG3, LGALS2, LGALS3, MAC 2 antigen, Mac-2, Mac-2 antigen, MAC2, Macrophage galactose-specific lectin, MGC105387,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified