Actinin α4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90025-50, BS90025-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43707(Human) P57780(Mouse) Q9QXQ0(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IF, IP, IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure. are actually sensitive to the Profilin proteins in these foods.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Actinin α4 polyclonal antibody detects endogenous levels of Actinin α4 protein.

Bioworld Molecular Weight: 105 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: NIH-3T3
Lane 4: Rat liver tissue
Lane 5: A431
Lane 6: HepG2, ICC staining alpha Actinin 4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ACTN4, actinin 4, Actinin alpha 4, actinin4, ACTN 4, ACTN4, ACTN4, Alpha-actinin-4, DKFZp686K23158, F actin cross linking protein, F-actin cross-linking protein, Focal segmental glomerulosclerosis 1, FSGS 1, FSGS, FSGS1, Non muscle alpha actinin 4, Non-muscle alpha-actinin 4,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified