EB3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90441-50, BS90441-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UPY8(Human) Q6PER3(Mouse) Q5XIT1(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: EB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigenesis of colorectal cancers and proliferative control of normal cells. EB1 may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Colorectal cancer is caused by the pathologic transformation of normal colonic epithelium to an adenomatous polyp, which can become an invasive cancer. APC (adenomatous polyposis coli) is a tumor suppressor gene, the mutation of which is one of the earliest events in colorectal carcinogenesis. A majority of the mutations result in the loss of the carboxy terminus of APC. EB1 has been shown to bind to the carboxy terminal region of APC, which implicates EB1 in APC suppression of colonic cancer. EB1 overexpression may play a role in the development of human esophageal squamous cell carcinoma (ESCC) by affecting APC function and activating the beta-catenin/TCF pathway. EB3 is related to EB1 and likewise associates with the microtubule cytoskeleton. EB3 is expressed predominantly in the central nervous system and preferentially associates with APCL.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: EB3 polyclonal antibody detects endogenous levels of EB3 protein.

Bioworld Molecular Weight: 32 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain tissue
Lane 2: K562, ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: EBF3, EB3, MAPRE3, APC binding protein, EB 3, EB1 protein family member 3, EB3, EBF 3, EBF3, EBF3 S, End binding protein 3, End-binding protein 3, MAPRE 3, MAPRE3, MARE3, Member 3, Microtubule Associated Protein, Microtubule associated protein RP/EB family member 3, Microtubule-associated protein RP/EB family member 3, OTTHUMP00000200910, RP 3, RP/EB Family, RP3,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified