YB1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12308-50, MB12308-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P67809

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 36 kDa; Observed MW: 49 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of YB1 in 3T3 lysates using YB1 antibody., Western blot analysis of YB1 in Hela, C2C12 lysates using YB1 antibody., Immunohistochemistry analysis of paraffin-embedded Human colon cancer using YB1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunocytochemistry analysis of YB1 in hela using YB1 antibody, and DAPI.

Alternative Name: YBX1; NSEP1; YB1; Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-

Immunogen: A synthetic peptide of human YB1

Conjugate: Unconjugated

Modification: Unmodified