eIF4E Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10869-50, MB10869-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P06730

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

Background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 25 kDa; Observed MW: 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of eIF4E in HCT116 using eIF4E antibody, and DAPI., Western blot analysis of eIF4E in HL-60 lysates using eIF4E antibody., Western blot analysis of eIF4E in C6, 3T3 lysates using eIF4E antibody

Alternative Name: EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Immunogen: A synthetic peptide of human eIF4E

Conjugate: Unconjugated

Modification: Unmodified