WT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91456-50, BS91456-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P19544

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF, IHC ELISA

All Applications: WB: 1:500~1:2000
IHC: 1:100~1:500
IF: 1:100~1:500
ELISA: 1:5000~1:10000

Background: Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino-terminus. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: WT1 polyclonal antibody detects endogenous levels of WT1 protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample: TM4 Cell (Mouse) Lysate at 40 ug
Primary: Anti-WT1 at 1/300 dilution, Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-WT1 at 1/300 dilution, Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-WT1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WT1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

Alternative Name: WIT 2; WT 1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms Tumor; Wilms tumor protein; Wilms' tumor gene; Wilms' tumor protein; WIT2; WT; WT1; WT-1; WT1_HUMAN; WT33

Immunogen: KLH conjugated synthetic peptide derived from human WT1: 301-400/449

Conjugate: Unconjugated

Modification: Unmodified